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主营:分子类,蛋白类,抗体类,生化类试剂
℡ 4000-520-616
℡ 4000-520-616
MD Biosciences/Chondroitinase generated C-4-S & DS Antibody/100 ug/1042009
产品编号:1042009
市  场 价:¥0.00
场      地:美国(厂家直采)
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:待定
品      牌: MD Biosciences
公      司:MDBiosciences
公司分类:
MD Biosciences/Chondroitinase generated C-4-S & DS Antibody/100 ug/1042009
商品介绍
  • Overview
  • Data/Specifications
  • Literature/Support
  • How To Use
  • Related Products

Overview

Overview:

Monoclonal antibody to delta-unsaturated glucuronic acid adjacent to N-acetylgalactosamine- 4-sulphate in the non-reducing terminal dissacharide “stub” of 4-sulphated chondroitin sulfate that is produced after chondroitinase digestion of chondroitin-4- sulphate glycosaminoglycan chains (chondroitinase ABC or ACII) or dermatan sulphate glycosaminoglycan chains (chondroitinase ABC or chondroitinase B).

Related Terms/Symbols:

  • Chondroitin-4-sulfate
  • C-4-S
  • Dermatan-sulfate

Data/Specifications

Data/Specifications:

Immunogen: Bovine nasal cartilage aggrecan that had been deglycosylated with chondroitinase ABCdigestion; i.e. BNC (ABC) Core (see Couchman et al 1984 and Caterson et al 1985).

Clone: 2B6

Isotype: IgG

Host: Mouse

Purity: Affinity purified

Specificity: Chondroitin-4-sulphate (C-4-S) after treatment with Chondroitinase ABC or ACII. Dermatansulphate (DS) neoepitope after treatment with Chondroitinase ABC or B.

Form: liquid

Storage: -20° C

Literature/Support

Literature/Support/Publications:

Antibody to Chondroitinase-generated C-4-S and DS neoepitope Insert (PDF, 338 KB)

Joint Disease and Aggrecan (blog post)

Caterson, B, Christner, JE, Baker, JR, Couchman, JR. (1985) Production and character- ization of monoclonal antibodies directed against connective tissue proteoglycans Fed Proc 44,386-393
Couchman, J.R., Caterson, B., Christner, J.E. and Baker, J.R. (1984). Mapping by Monoclonal Antibody Detection of Glycosaminoglycans in Connective Tissues. Nature 307: 650-652
Rees SG, Flannery CR, Little CB, Hughes CE, Caterson B & Dent CM (2000). Catabolism of aggrecan, decorin and biglycan in tendon. Biochem J. 350: 181-188.
Hayes AJ, Hall A, Brown L, Tubo R & Caterson B (2007). Macromolecular organization and in vitro growth characteristics of scaffold-free neocartilage grafts. J Histochem Cytochem. 55(8):853-66.
Hayes AJ, Hughes CE & Caterson B (2008). Antibodies and immunohistochemisrty in extracellular matrix research. Methods 45: 10-21
Stefan Milz, Frank Regner, Reinhard Putz, and Michael Benjamin. Expression of a Wide Range of Extracellular Matrix Molecules in the Tendon and Trochlea of the Human Superior Oblique MuscleInvestigative Ophthalmology and Visual Science. 2002;43:1330-1334
Hedlund, H, Hedbom, E, Heinegard, D, Mengarelli-Widholm, S, Reinholt, FP, Svensson, O. (1999) Association of the aggrecan keratan sulfate-rich region with collagen in bovine articular cartilage J Biol Chem 274,5777-5781

Caterson, B, Christner, JE, Baker, JR, Couchman, JR. (1985) Production and character- ization of monoclonal antibodies directed against connective tissue proteoglycans Fed Proc 44,386-393

Couchman, J.R., Caterson, B., Christner, J.E. and Baker, J.R. (1984). Mapping by Monoclonal Antibody Detection of Glycosaminoglycans in Connective Tissues. Nature 307: 650-652

Rees SG, Flannery CR, Little CB, Hughes CE, Caterson B & Dent CM (2000). Catabolism of aggrecan, decorin and biglycan in tendon. Biochem J. 350: 181-188.

Hayes AJ, Hall A, Brown L, Tubo R & Caterson B (2007). Macromolecular organization and in vitro growth characteristics of scaffold-free neocartilage grafts. J Histochem Cytochem. 55(8):853-66.

Hayes AJ, Hughes CE & Caterson B (2008). Antibodies and immunohistochemisrty in extracellular matrix research. Methods 45: 10-21

Stefan Milz, Frank Regner, Reinhard Putz, and Michael Benjamin. Expression of a Wide Range of Extracellular Matrix Molecules in the Tendon and Trochlea of the Human Superior Oblique MuscleInvestigative Ophthalmology and Visual Science. 2002;43:1330-1334

Hedlund, H, Hedbom, E, Heinegard, D, Mengarelli-Widholm, S, Reinholt, FP, Svensson, O. (1999) Association of the aggrecan keratan sulfate-rich region with collagen in bovine articular cartilage J Biol Chem 274,5777-5781

How To Use

How To Use:

Applications

  • ELISA
  • Western Blot
  • Immunohistochemistry

Technical Notes

Pretreatment with Chondroitinase required. 1x10-3 U per 10 mg GAG at 37 °C for 1 hour.

Related Products

品牌介绍

MD Biosciences成立于1991年,总部位于瑞士,在美国、加拿大和英国设有办事处,是一家领先的研发和临床阶段的制药和生物技术的公司,提供尖端的研究服务,创新的生物产品和先进的诊断能力。我们在炎症和神经学领域拥有超过25年的无与伦比的科学专业知识和专有方法历史,这些使我们一直处于精确医学运动的前沿。

 

其主要产品包括:

抗体  (软骨蛋白聚糖、胶原蛋白、激素、神经药物)、细胞培养相关试剂  (胶原蛋白、细胞增殖检测、支原体PCR检测)、疾病诱导试剂  (关节炎诱导抗体混合、弗氏佐剂、胶原蛋白II)ELISA试剂盒(糖蛋白、细胞因子、激素等)、同型对照、天然蛋白以及重组蛋白(胶原类等)。


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