- Overview
- Data/Specifications
- Literature/Support
- How To Use
- Related Products
Overview
Type I collagen is the most abundant collagen and is found in connective tissues including tendon, ligament, dermis and blood vessel. It is the major component and the primary determinant of tensile strength of the extracelluar matrix (ECM). It is widely used as a thin layer on tissue-culture surfaces to enhance the attachment and proliferation of a variety of cells including endothelial cells, fibroblasts, hepatocytes, epithelial cells etc. In addition, collagen I can self-assemble into a 3-D superamolecular gel in vitro, making it an ideal biological scaffold to promote more in vivo-like cellular morphology and function.
Symbols/Related Terms:
- COL1A1
- COL1A2
- osteogenesis imperfecta
Data/Specifications
Description: Purified Mouse Collagen Type I (Atelocollagen) from mouse tail tendon
Form: Lyophilized, salt free, 1 mg/ vial
Sterility: Passed sterility test for bacteria and fungi
Source: Mouse tail tendons
Purity:Mouse collagen type I > 90% / Mouse collagen type III < 10%
Purification:Collagen was extracted from washed dissected tissue into diluted acetic acid after mild pepsin treatment. Collagen type I was purified by using differential salt precipitation.
Reconstitution: Dissolve in 20 mM acetic acid. Suspension should be shaken or stirred for several hours or overnight between 2-8 °C. Material is dissolved when it appears homogeneous, with no schlieren pattern (light refraction due to differing protein concentrations in the suspensions). Recommended concentration 1-5 mg/mL.
Storage: Collagen dissolved in acetic acid is stable at 4 °C for 1 month. Lyophilized collagen long term storage at -20 °C or lower.
Applications: Cell culture (coating or 3D gel)
Literature/Support
Purified Mouse Collagen Type I (Atelocollagen) from mouse tail tendon INSERT (PDF)
Note: inserts are for review only. Please use the insert that was sent with the product to ensure that the correct revision insert is being used for the product purchased.
How To Use
Dissolve in 20 mM acetic acid. Suspension should be shaken or stirred for several hours or overnight between 2-8°C. Material is dissolved when it appears homogeneous, with no schlieren pattern (light refraction due to differing protein concentrations in the suspensions). Recommended concentration 1-5 mg/mL.
Related Products
MD Biosciences成立于1991年，总部位于瑞士，在美国、加拿大和英国设有办事处，是一家领先的研发和临床阶段的制药和生物技术的公司，提供尖端的研究服务，创新的生物产品和先进的诊断能力。我们在炎症和神经学领域拥有超过25年的无与伦比的科学专业知识和专有方法历史，这些使我们一直处于精确医学运动的前沿。
其主要产品包括：
抗体 (软骨蛋白聚糖、胶原蛋白、激素、神经药物)、细胞培养相关试剂 (胶原蛋白、细胞增殖检测、支原体PCR检测)、疾病诱导试剂 (关节炎诱导抗体混合、弗氏佐剂、胶原蛋白II等)、ELISA试剂盒（糖蛋白、细胞因子、激素等）、同型对照、天然蛋白以及重组蛋白（胶原类等）。
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