- Overview
- Data/Specifications
- Literature/Support
- How It Works
Overview
The need for a reliable, sensitive and quantitative assay that would enable analysis of a large number of samples led to the development of methods, such as:
- use of radioactive thymidine to label DNA in live cells
- use of Brdu to label DNA in live cells (as a substitute for radioactive thymidine)
The advantages of using MD Bioproducts Cell Proliferation Kit (XTT) can be summarized with the following attributes:
- easy-to-use: there is no requirement for additional reagents and/or the cell washing procedures
- speed: multiwell plates and an ELISA reader can be used for reading
- sensitivity: can be assayed even in low cell concentrations
- accuracy: dye absorbance is proportional to the number of cells in each well
- safety: there is no need for radioactive isotopes
Data/Specifications
- Kit Components
- XTT Reagent
- Activation Reagent
Literature/Support
Cell Proliferation Kit Data Insert (PDF, 439 KB)
Nagafuku, M., Okuyama, K., Onimaru, Y., Suzuki, A., Odagiri, Y., Yamashita, T. & Inokuchi, J. I. (2012). CD4 and CD8 T cells require different membrane gangliosides for activation. Proceedings of the National Academy of Sciences, 109(6), E336-E342.
How It Works
The assay is based on the ability of metabolic active cells to reduce the tetrazolium salt XTT to orange colored compounds of formazan. The dye formed is water soluble and the dye intensity can be read at a given wavelength with a spectrophotometer. The intensity of the dye is proportional to the number of metabolic active cells. The use of multiwell plates and an ELISA reader enables testing a large number of samples and obtaining easy and rapid results. The test procedure includes cultivation of cells in a 96-well plate, adding the XTT reagent and incubation for 2-24 hours. During incubation an orange color is formed, the intensity of which can be measured with a spectrophotometer, in this instance with an ELISA reader. The greater the number of active cells in the well, the greater the activity of mitochondria enzymes, and the higher the concentration of the dye formed, which can then be measured and quantitated.
MD Biosciences成立于1991年，总部位于瑞士，在美国、加拿大和英国设有办事处，是一家领先的研发和临床阶段的制药和生物技术的公司，提供尖端的研究服务，创新的生物产品和先进的诊断能力。我们在炎症和神经学领域拥有超过25年的无与伦比的科学专业知识和专有方法历史，这些使我们一直处于精确医学运动的前沿。
其主要产品包括：
抗体 (软骨蛋白聚糖、胶原蛋白、激素、神经药物)、细胞培养相关试剂 (胶原蛋白、细胞增殖检测、支原体PCR检测)、疾病诱导试剂 (关节炎诱导抗体混合、弗氏佐剂、胶原蛋白II等)、ELISA试剂盒（糖蛋白、细胞因子、激素等）、同型对照、天然蛋白以及重组蛋白（胶原类等）。
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