- Overview
- Data/Specifications
- Literature/Support
- How To Use
- Related Products
Overview
Aggrecan is a member of a family of large, aggregating proteoglycans (also including versican, brevican and neurocan) which is found in articular cartilage. Aggrecan is composed of three major domains: G1, G2, and G3. Between the G1 and G2 domains there is an interglobulin region (IGD). The IGD region is the major site of cleavage by specific proteases like metalloproteinases (MMPs) and aggrecanase. Aggrecan cleavage has been associated with a number of degenerative diseases including rheumatoid arthritis and osteoarthritis. There is evidence that this family of proteoglycans modulates cell adhesion, migration, and axonal outgrowth in the CNS.
Data/Specifications
Immunogen:NITEGE synthetic peptide conjugate.
Clone: BC-13
Host: Mouse
Myeloma: x63-Ag8.653
Isotype: IgG1
Light Chain type: kappa
Specificity:Recognizes the aggrecanase (ADAMTS-1, -4 & -5)-generated C-terminal neoepitope ...TEGE after cleavage between amino acids ..EGE373 and 374ARG.. within the interglobular domain of aggrecanase-catabolised aggrecan (Human aggrecan sequence enumeration).
Cross-reactivity:Human, Cow, Dog, Horse, Pig.
Purity: Affinity purified on protein G
Form: Liquid, 1 mL/vial
Concentration: 0.1 mg/mL
Storage: -20° C
Literature/Support
Aggrecan Antibody, clone BC-13 Insert (PDF, 334 KB)
Joint Disease and Aggrecan (blog post)
Ashinsky, B. G., Gullbrand, S. E., Bonnevie, E. D., Mandalapu, S. A., Wang, C., Elliott, D. M., ... & Smith, H. E. (2019). Multiscale and multimodal structure–function analysis of intervertebral disc degeneration in a rabbit model. Osteoarthritis and Cartilage, 27(12), 1860-1869.
How To Use
Applications
- Western Blot -Suggested dilution: 1:100.Detects a a band of approximately 60-70 kDa
- ELISA
- IHC
Technical Notes
Samples are usually deglycosylated using 0.01 Units chondroitinase ABC (Sigma), 0.01
Units Keratanase (Seikagaku) and 0.0001 Units Keratanase II (Seikagaku) per 10μg S-GAG
of non-deglycosylated aggrecan for optimal epitope recognition in SDS-PAGE and immunohistochemistry
(1, 2).
Related Products
MD Biosciences成立于1991年，总部位于瑞士，在美国、加拿大和英国设有办事处，是一家领先的研发和临床阶段的制药和生物技术的公司，提供尖端的研究服务，创新的生物产品和先进的诊断能力。我们在炎症和神经学领域拥有超过25年的无与伦比的科学专业知识和专有方法历史，这些使我们一直处于精确医学运动的前沿。
其主要产品包括：
抗体 (软骨蛋白聚糖、胶原蛋白、激素、神经药物)、细胞培养相关试剂 (胶原蛋白、细胞增殖检测、支原体PCR检测)、疾病诱导试剂 (关节炎诱导抗体混合、弗氏佐剂、胶原蛋白II等)、ELISA试剂盒（糖蛋白、细胞因子、激素等）、同型对照、天然蛋白以及重组蛋白（胶原类等）。
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