- Overview
- Data/Specifications
- Literature/Support
- How To Use
- Related Products
Overview
During differentiation of neural precursor cells, neurospheres downregulate Chondroitin sulfate proteoglycans (CSPGs). Proliferating neural precursors synthesize lecticans, including aggrecan, which are downregulated with differentiation; suggesting a link between CSPGs and CNS precursor biology.
Data/Specifications
Immunogen:ARGSV synthetic peptide conjugate. Clone: BC-3 Host: Mouse Myeloma: x63-Ag8.653 Isotype: IgG1 Light Chain type: kappa Specificity:Recognizes the aggrecanase (ADAMTS-1, -4 & -5)-generated N-terminal neoepitope ARG after cleavage between amino acids EGE and ARG within the interglobular domain of aggrecanase-catabolised aggrecan (Human aggrecan sequence enumeration). This antibody will not recognize the sequence ARG if it is in the non-cleaved intact aggrecan protein core; i.e. it will only recognize the aggrecanase generated neoepitope ARG. Cross-reactivity:human, rat, cat, cow, dog, guinea pig, horse, pig, rabbit and sheep. Purity: Affinity purified on protein G Form: Liquid, 1 mL/vial Concentration: 0.1 mg/mL Storage: -20° C |
Literature/Support
Aggrecan antibody, clone BC-3 Insert (PDF, 335 KB)
Joint Disease and Aggrecan (Blog Post)
How To Use
Applications
- Western Blot -Suggested dilution: 1:100.Detects a variety of epitopes between 50 and 250 kDa
- ELISA
- IHC with frozen sections or formalin/PFA-fixed paraffin embedded sections
Technical Notes
Samples must be deglycosylated using 0.01 Units Chondroitinase ABC (Sigma), 0.01 Units
Keratanase (Seikagaku) and 0.0001 Units Keratanase II (Seikagaku) per 10μg S-GAG of nondeglycosylated
aggrecan for optimal epitope recognition.
Related Products
MD Biosciences成立于1991年，总部位于瑞士，在美国、加拿大和英国设有办事处，是一家领先的研发和临床阶段的制药和生物技术的公司，提供尖端的研究服务，创新的生物产品和先进的诊断能力。我们在炎症和神经学领域拥有超过25年的无与伦比的科学专业知识和专有方法历史，这些使我们一直处于精确医学运动的前沿。
其主要产品包括：
抗体 (软骨蛋白聚糖、胶原蛋白、激素、神经药物)、细胞培养相关试剂 (胶原蛋白、细胞增殖检测、支原体PCR检测)、疾病诱导试剂 (关节炎诱导抗体混合、弗氏佐剂、胶原蛋白II等)、ELISA试剂盒（糖蛋白、细胞因子、激素等）、同型对照、天然蛋白以及重组蛋白（胶原类等）。
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