- Overview
- Data/Specifications
- Literature/Support
- How To Use
- Related Products
Overview
Proteoglycans are categorized depending upon the nature of their glycosaminoglycan chains (chondroitin sulfate, dermatan sulfate, heparan sulphate and keratan sulfate) as well as characterized by size.
Aggrecan is a large aggregating proteoglycan of articular cartilage. It is also found in aorta tissue, discs, tendons and in the perineuronal net. It is responsible for hydrating cartilage, giving it compressibility and resilience during joint loading, thereby playing a major role in the normal function of cartilage. Depletion of glycosaminoglycan-bearing aggrecan fragments is one of the earliest events in cartilage destruction.
Data/Specifications
Immunogen:Synthetic peptide: EPEEPFTFAPEI
Clone:6B4
Host: Mouse
Myeloma: x63-Ag8.653
Isotype: IgG1
Light Chain type: kappa
Specificity:Recognizes the linear amino acid sequence 394EPEEPFTFAPEI406 present in the interglobulardomain (IGD) of human and bovine aggrecan.
Cross-reactivity: This antibody cross-reacts with human and bovine
Purity: Affinity purified on protein G
Form: Liquid, 1 mL/vial
Concentration: 0.1 mg/mL
Storage: -20° C
Literature/Support
Aggrecan IGD Antibody Insert (PDF, 334 KB)
Joint Disease and Aggrecan (blog post)
How To Use
Applications
- ELISA: Use at an assay dependant dilution.
- IHC-P: Use at an assay dependant dilution.
- IHC-Fr: Use at an assay dependant dilution.
- WB: Use at a dilution of 1/100.
Technical Notes
Optimal dilutions/concentrations should be determined by the end user. This antibody
should work in IHC on formalin or paraformaldehyde-fixed paraffin embedded sections as
well as either alcohol-fixed frozen sections or un-fixed snap-frozen sections.
Samples are usually deglycosylated using 0.01 Units Chondroitinase ABC (Sigma), 0.01
Units Keratanase (Seikagaku) and 0.0001 Units Keratanase II (Seikagaku) per 10ug S-GAG
of non-deglycosylated aggrecan for optimal epitope recognition in SDS-PAGE and immunohistochemistry.
Related Products
MD Biosciences成立于1991年，总部位于瑞士，在美国、加拿大和英国设有办事处，是一家领先的研发和临床阶段的制药和生物技术的公司，提供尖端的研究服务，创新的生物产品和先进的诊断能力。我们在炎症和神经学领域拥有超过25年的无与伦比的科学专业知识和专有方法历史，这些使我们一直处于精确医学运动的前沿。
其主要产品包括：
抗体 (软骨蛋白聚糖、胶原蛋白、激素、神经药物)、细胞培养相关试剂 (胶原蛋白、细胞增殖检测、支原体PCR检测)、疾病诱导试剂 (关节炎诱导抗体混合、弗氏佐剂、胶原蛋白II等)、ELISA试剂盒（糖蛋白、细胞因子、激素等）、同型对照、天然蛋白以及重组蛋白（胶原类等）。
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