- Overview
- Data/Specifications
- Literature/Support
- How To Use
- Related Products
Overview
Type I collagen is the most abundant form of collagen in the human body and is synthesized mainly by fibroblasts, osteoblasts, odontoblasts and chondroblasts.It is located in the extracellular matrix of many tissues of the body including cartilage, bone, tendon, skin and the sclera of the eye.Type I collagen is composed of two pro-α1(I) chains, produced from the COL1A1 gene, and one pro-α2(I) chain, produced from the COL1A2 gene.Mutations in the genes that produce collagen type I are responsible for causing various health conditions including Ehlers-Danlos syndrome, osteogenesis imperfecta, osteoporosis and Caffey disease.
Data/Specifications
Format: IgG fraction, freeze-dried (0.5 ml). Reconstitute with 0.5 mldi H2O and store aliquots at -20°C.
Specificity (% at 1:200 RIA dilution):
Rat Collagen, Type I : 100%
Rat Collagen, Types II, III, V : < 0.1%
Human, mouse, chicken Collagen Type I: < 0.1%
Rat elastin: <0.1%
Literature/Support
Anti-Rat Collagen Type I Antibody Insert (PDF, 283 KB)
References:
Dagher, P. C., Hato, T., Mang, H. E., Plotkin, Z., Richardson, Q. V., Massad, M., ... & Sutton, T. A. (2016). Inhibition of toll-like receptor 4 signaling mitigates microvascular loss but not fibrosis in a model of ischemic acute kidney injury.International journal of molecular sciences,17(5), 647.
Sutton, T. A., Hato, T., Mai, E., Yoshimoto, M., Kuehl, S., Anderson, M., ... & Dagher, P. C. (2013). p53 is renoprotective after ischemic kidney injury by reducing inflammation.Journal of the American Society of Nephrology,24(1), 113-124.
Dagher, P. C., Mai, E. M., Hato, T., Lee, S. Y., Anderson, M. D., Karozos, S. C., ... & Sutton, T. A. (2011). The p53 inhibitor pifithrin-α can stimulate fibrosis in a rat model of ischemic acute kidney injury.American Journal of Physiology-Renal Physiology,302(2), F284-F291.
How To Use
For immunostaining of extra or intracellular components inlight microscopy and the quantitation of Collagen Type I inbiological fluids or in culture supernatants.
Applications
- IFA
- IHC(p)
- (SP) RIA
- ELISA
Working Dilutions
- Indirect IFA (with fluoresceine anti-rabbit IgG conjugate): >1:80 on frozen rat tissues (liver, skin...).
- IHC(p): >1:500 on fixed, paraffin-embedded rat tissues
- ELISA: >1:200 (OD <500)
Related Products
MD Biosciences成立于1991年，总部位于瑞士，在美国、加拿大和英国设有办事处，是一家领先的研发和临床阶段的制药和生物技术的公司，提供尖端的研究服务，创新的生物产品和先进的诊断能力。我们在炎症和神经学领域拥有超过25年的无与伦比的科学专业知识和专有方法历史，这些使我们一直处于精确医学运动的前沿。
其主要产品包括：
抗体 (软骨蛋白聚糖、胶原蛋白、激素、神经药物)、细胞培养相关试剂 (胶原蛋白、细胞增殖检测、支原体PCR检测)、疾病诱导试剂 (关节炎诱导抗体混合、弗氏佐剂、胶原蛋白II等)、ELISA试剂盒（糖蛋白、细胞因子、激素等）、同型对照、天然蛋白以及重组蛋白（胶原类等）。
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